Cancer Cell Line Encyclopedia Metabolomics

The cancer cell line encyclopedia is a database of gene expression genotype and drug sensitivity data for human cancer cell lines.

Cancer cell line encyclopedia metabolomics. A compilation of gene expression chromosomal copy number and massively parallel sequencing data from 947 human cancer cell lines. 2012 the cancer cell line encyclopedia enables predictive modelling of anticancer drug sensitivity. In this publication we expand the cancer cell line encyclopedia to include quantitative proteome profiling by mass spectrometry of 375 cell lines from diverse lineages. The experiment was performed in multiplex format with 9 biological samples per plex and one common sample to normalize between plexes.

Citation s barretina j et al. When coupled with pharmacological profiles for 24 anticancer drugs across 479 of the cell lines this collection allowed identification of genetic lineage and gene expression based predictors of drug sensitivity. To further understand the metabolic diversity of cancer we profiled 225 metabolites in 928 cell lines from more than 20 cancer types in the cancer cell line encyclopedia ccle using liquid chromatography mass spectrometry lc ms. Since the whole body participates in the metabolic process and each cell releases its own metabolites many factors can influence the results of metabolomics raw data29 such as gender 30 drug usage3 and dietary habits 31 african american or caucasian women have shown different metabolic profiles of triple negative breast cancer tnbc 32 these.

2020 the broad institute of mit harvard. Nmr based metabolomics have also been used to identify biomarkers for cancer including hepatocellular carcinoma hcc colon cancer ovarian cancer breast cancer prostate cancer colorectal cancer and others 4 35 36 37 38 39 40 41. Factors influencing metabolomics raw data. Here we describe the cancer cell line encyclopedia ccle.